茚三酮比色法测定CMA馏出物中氨基酸含量的研究.pdf

第3期
化学世界
茚三酮比色法测定CMA留出物中氨基酸含量的研究
王丽萍2,姜久兴2,许英梅·,王军华,权春善',祁恩云”,范圣第
(1.大连民族学院生物化工.国家民委-教育部重点实验室,生命科学学唳,大连116600
2,哈尔滨理工大学,应用科学学院,黑龙江哈尔滨150080)
摘要;采用茚三酮比色法对木醋液制备环保型融雪剂过程中的馏出物中氨基酸进行分析,结果表
明,馏出物中魚基酸的含量约为1.4g/T,且不受活性炭用量的影响,馏出物应调整到弱酸性条件
(pI=4左右),才能保证稳定的氪基酸含量。研究结果可为植物生长促进剂的开发提供理论依
据,并将促进木醋液的综合利用和生物质能的开发
关键词:木酷液;基酸;茚三酮
中图分类号:)656文献标志码:A
文章编号:0367~-6358(2011)03-0141-03
Quantification of Amino Acid in Distillate of CMA Deicer
Production through Ninhydrine Colorimetr
WANG Li-ping, JIANG Jiu-xing, XU Ying-mei, WANG Jun-hua
QI Er
QUAN Chun-sh
AN Sheng-di
1. Key Laboratory of Biochemical, The State Ethnic A fairs Comnmission-ministy of
Education, School of Biological Science of Dalian Nationalities Wizersty, Liconing Dalian 116600, China,
2. School of Applied Science of Harbin University of Science and Technology, Helongjiang Haerbin 150080, China)
Abstract The ninhydrine colorimetry was applied to quantify the amino acids of distillate in the process of
producing calcium magnesinm acetate which was an environmentally friendly deicer from wood vinegar.
The results indicated that the total content of amino acids was about 1. 4 g/L, and it was not influenced b
the amount of activated charcoal used in the experiment. In addition, it was also observed that the content
of amino acids was more stable under weak acidic condition(PH=4 or so). This research about amino acid
content in the distillate was helpful for the further development of plant growth promoters, comprehensive
utilization of wood vinegar and expanding the use of biomass energy.
Key words: wood vinegar; amino acid; ninhydrine
木醋液也叫植物酸,是生物质废料(木材、木屑、精制分离技术以及综合利用技术进行了研究1,其
稻壳、秸秆、板栗壳等)干馏热解后的气体产物经过中由木醋液制CMA类环保型融雪剂技术,为道路
冷凝得到的液体组分。木醋液作为生物质能开发利除冰雪提供了一种低腐蚀性的生物基融雪剂24
用的副产物,其综合利用直接影响产业发展和环境木醋液中含有200多种有机物,除了主要含有10%
经济效益,近年来,研究者围绕木醋液的组成、性质、左右的醋酸外,还含有多种氮基酸,对植物生长具有
促进作用?。在由木醋液制CMA类环保型融雪
收稿日期:2010-06-28;修回日期:2010-02-15
基金项国:辽宁省自然科学基金资助项目(20052199)
作者简介:许英梅(1964~),女,副教授,主要从事资源综合利用与绿色化学品教学和科研。 E-mail:xym@d
13840897526;0411-87656143。