高灵敏特异性红霉素印迹传感器用于血样中红霉素的测定.pdf

第43卷第3期第249页
华中科技大学学报(医学版)
Vol.43No.3P.249
2014年6月
Acta Med Uniy Sci Technol Huazho
Jun.2014
高灵敏特异性红霉素印迹传感器用于血样中
红霉素的测定
宋彬',周雨笋,荆涛,周廷廷1,刘敏,郝巧玲',周宜开',梅素容14
华中科技大学同济医学院公共卫生学院环境医学研究所,教育部环境与健康重点实验室,武汉430030
2华中科技大学化学与化工学院,武汉430074
摘要:目的构建一种高灵敏特昇性分子印迹电化学传感器用于血样中红霉素( erythromycin,ERY)的检测。
方法采用沉淀聚合法,以ERY为模板分子,优化模板分子与功能单体之间的摩尔比和交联剂的用量,制备一种分子印
迹聚合物( molecularly imprinted polymers,MIPs),并利用其特异识别能力与乙炔黑( acetylene black,AB)高催化活性相
结合,构建一种分子印迹电化学传感器,用于血样中ERY的检测。结果该传感器对ERY具有良好的选择性以及较高
的灵敏度,印迹因子为3.6。检测线性范围为1.0×10~8~5.0×10-6mol/L(ァ2=0.9978),检出限为4.3×10~9mol/L。
该法用于人血清中ERY的测定,加标回收率为82.0%~119.8%。结论建立了一种高灵敏特异性分子印迹电化学传
感器,能用于血样中FRY的快速检测,为指导FRY的使用和保护人体健康提供有力的技术支持。
关键词:红霉素;分子印迹聚合物;乙炔黑;碳糊传感器;血样
中图分类号:R446.39DOI:10.3870/j.iss.1672-0741.2014.03.002
Application of Highly Sensitive and Specific Erythromycin-imprinted Sensors
for Determination of Erythromycin in Blood Samples
Arng Bin, Zhou Yusun, Jing Tao et ai
Key Laboratory of Environment Health of Ministry of Education, Institute of Environmental Medicine, School o
Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Abstract Objective To construct a highly sensitive and specific molecularly imprinted electrochemical sensor for detecting
erythromycin (ERY) in blood samples. Methods After optimizing the molar ratio of the template molecule(ERY) to functional
monomer and the amount of cross-linker, molecularly imprinted polymers(MIPS) was prepared by precipitation
polymerization. Then, a molecularly imprinted electrochemical sensor was constructed by combining MIPS of specific recognition
bility with acetylene black(AB) of highly catalytic activity to detect ERY in blood samples. Results This sensor was highly
selective and sensitive for ERY, with the imprinting factor of 3. 6. The linear range of the sensor was 1.0X 10-5.0X10 6
mol /L(=0. 9978), with a limit of de ection(LOD) of 4. 3X 10 mol/L. The recovery of the spiked ERY in blood samples
was found to be from 82.0% to 119.8. Conclusion A highly sensitive and specific molecularly imprinted electrochemical sen
sor was constructed. The sensor can be used for rapidly detecting ERY in blood samples and is expected to provide technical
support for guidance of the use of ER Y and protection of human health
Key words erythromycin; molecularly imprinted polymers; acetylene black; carbon paste sensor; blood samples
红霉素( erythromycin,ERY)是一种被广泛应物代谢,进而影响这些药物在体内的分布,引起显著
用的大环内酯类抗生素,经常用于对青霉素过敏人的临床副反应。因此,ERY快速、灵敏检测方法
群的抗感染作用。进人体内的ERY能够被迅速的建立能够为指导用药和保护人体健康提供技术支
吸收入血、分散在组织和吞噬细胞中2。然而,无论持,具有重要的应用价值和现实意义。目前,ERY
口服或静脉注射ERY都存在一些不良反应,有可的分析方法主要包括高效液相色谱法们、高效液相
能诱导患者的室性心动过速和心律不齐,还能够通串联质谱法7』、近红外光谱法可、毛细血管电泳"?
过钝化细胞色素氧化酶P450的活性,抑制多种药及电化学法等。其中,电化学方法具有简便、成
“新世纪优秀人才计划(No.NCET-1-0178),教育部博土点基金
本低、分析时间短和灵敏度高的优点,同时ERY又
(No.221201421002),武汉市科技攻关计划资助项目(N.2013具有电化学活性,因此利用电化学方法检测ERY
020501010181
宋彬,男,1987年生,医学硕上,F-mail: hustsongbint126,com
是一种理想的选择。然而,传感器的敏感元件缺乏
通讯作者, Corresponding author, E-rnail: surongmeit@hust.ecdu.en
时ERY的选择识别能力是电化学分析方法面临的