俊品与酵科技
Food and Fermentation Teehnology
第51卷(第3期)Vol.57,N
异养小球藻
培养基优化筛选
王方方,杜风光,刘铖,徐灵鹤,张鹏飞,姜超
河南天冠企业集团有限公司,河南南阳473000
摘要:异养小球藻生长周期较长,需要改进培养条件来提高效率通过将异养小球藻接种到异养小球藻培养基和其
它常用培养基用于对比培养后发现,氮含量高、氮源种类丰富的培养基可以有效促进异养小球藻生物量的增长。对异
养小球藻培养基进行优化、补充氮源后,对比培养,发现改良后的异养小球藻培养基质量优于原有培养基。新培养基
用于500.小试经过10d培养,细胞数最高值达到21.0亿,质量浓度达到126.3g.,与原有培养基相比,发酵周期缩短
5d.改良培养基对异养小球藻的细胞数与质量浓度增长最为有利
关键词:异养小球藻;培养基;优化
中图分类号:TQ920.1
文献标识码
文章编号:1674-506X(2015)03-0023-0004
Medium Optimization and Screening of Heterotrophic chlorella
WANG Fang-fang, DU Feng-guang, LIU Yue, ZHANG Peng-fei, XU Ling-he, JIANG Chao
Henon Tianguan Group o, A.., Nonyung Henon 473000, Chine
Abstract: It's necessary to improve the cult
uire conditons
of Heterotrophic chlorella in order to enhance efficieney
because its growth cycle was too long. After culture comparison of Heterotrophic chlorella between Heterotrophic
chlorella medium and other commonly used mcdium, it was found that the medium contained high nitrogen and
rich nitrogen sources can effeetively promote the growth of Heterotrophic chlorella biomass; After the optimization of
nal culture medium by supplement of nitrogen source, it was concluded that the optimized Heterotrophic
chlorella medium was better than the original medium when the new medium was used in 50L laboratory trial. the
cell population could reach 21.0 hundred million and the mass concentration could reach 126.3g/ after10 days
culture, meanwhile, the growth cell could decrease 5 days. Improved medium was the most favorable for the growth
of chlorella cell number and mass concentration
Key words: Heterotrophic chlorella; medium; optimization
doi:10.3969/.issn.1674-506X.2015.03-0
在当今能源危机与环保压力下,以生物质为原
生物柴油最初由植物油或动物脂肪衍生而来。
料生产的生物柴油,不需要添设加油、储存设备及人近年来,研究发现生物柴油也可由异养小球藻油脂
员的特殊技术训练,具有很大的发展潜力。生物柴油通过化学反应与短链醇,主要是甲醇,进行转酯反应
( biodiesel)具有无毒、可降解、可再生的特性,它的制备而成?
使用可以减少对传统化石燃料的消耗,从而滅少温
在所有生物柴油原料中,异养小球藻( heterotrophic
室气体和大气污染气体如硫化物、氮的氧化物的排 chlorella)具有较高的油脂生产率?,是油料作物(如
放,具有许多优于传统石油的特点2。
大豆)的30-100倍,并且在生长积累油脂过程中
收稿日期:2015-04-17
基金项目;能源微藻育种与高效生产关鍵技术研究与示范(2011BAD14B00)
作者简介:王方方(1983-),女,硕土研究生。主要从事菌种的保藏与筛选
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