同步荧光法结合偏最小二乘法对老陈醋中掺杂食用冰醋酸的定量分析.pdf

食品工程
2013年第3期
FOOD ENGINEERING
9月出版
同步荧光法结合偏最小二乘法
对老陈醋中掺杂食用冰醋酸的定量分析
Synchronous fluorescence spectrometry combined with partial least squares
method for quantitative analysis of edible acetic acid in adulterated
shanxi mature vinegar
郭洁丽”毛立新“杨
(山西大学生命科学学院,山西太原03000
UO Jie-li"MAO
(College of life science, Shaxi univerity, Shaxi Taiyuan 030006, China)
摘要采用偏最小二乘法(PIS)建立了山西老陈酷中摻杂食用冰醋酸的同步荧光定量检測模型。配制
不同体积比的食用冰醋酸和4种老陈醋混合样品共124个,其中每种醋样21个校正集,10个预测集。在
激发波长为250nm~550nm,波长间隔为70nm处收集同步荧光信号。采用交互验证方法进行建模,并使用
所建模型对未知样品进行预测。研究结果表明,相对偏差在5%之间,预测结果准确可靠,预测最低掺假
曼可达10%。因此,利用同步荧光技术结合偏最小二乘法检測老陈醋中食用冰酷酸的掺杂量是可行的。
关键词同妙荧光;偏最小二乘法;老陈酷;掺假;食用冰酷酸
Abstract By partial ]east squares(PLS )and synchronous fluorescent, quantitative detection model was established for
edible acetic acid in adulterated Shanxi mature vinegar. 124 kinds of mixed samples of edible acetic acid and four
kinds of mature vinegar were compounded, in which each of vinegar has 21 calibration sets and 10 validation sets S-
chronous fluorescence signal was collected in the 250 nm 550 nm of excitation wavelength and 70 nm of wavelength
interval Model was created by using cross-validation method. The results show that predicting the unknown samples by
the built model indicate that the relative deviation was5% the predicted results were accurate and reliable, the forecast
of minimum adul erant amounts was up to 10%. Therefore, deteting edible ac tir acid in adulterated Shani mature
vinegar by synchronous fluorescence technology combined with partial least squares is feasible
Keywords synchronous fluorescence; partial least squares; mature vinegar; adulteration; edible acetic acid
中图分类号:TYS
文献标识码:A
文章编号:1673-6004(2013)03-0048-04
DOI:10.3969/,isan.1673-604.2013.03.017
老陈醋是人们经常食用的酸性谓味品,不仅具可防治多种疾病,如具有软化血管、降低血压、
有调味功能,还含有丰富的营养物质。经常食用醋预防动脉硬化、治疗糖尿病等保健功能。山西老陈
*基金项目:国家自然科学基金资助项目(311717498);山西醋最大特色就在于制作工艺中的“陈",新醋一般
省科技攻关计划资助项目(2006031085-01)。
要经过1a~5a的陈酿,才能成为真正的老陈醋。
*郭洁丽,文,1989年出生,山西大学食品科学与工程专业目前一些不法商家为了降低成本、缩短生产周期,
在读研究生。
添加冰醋酸和色素等添加剂进行勾兑来代替粮食酿
**毛立新,通讯作者,E-mail:mx1963@163.com
造,使勾兑醋摇身一变成了老陈醋。
收稿日期:2013-08-19
同步荧光是以相同的扫描速度同时进行扫描,
万方数据