固定反硝化菌强化人工湿地处理低污染水研究.pdf

2016,35(11):2154-2162
农业环境科学学报
2016年11月
Journal of Agro-environment Science
林燕,张焕杰,刘曦,等.固定反硝化菌强化人工湿地处理低污染水研究叮农业环境科学学报,2016,35(11):2154-2162
LIN Yan, ZHANG Huan-jie IU Xi et al Performance of immobilized deni rif ing bacteria in constructed wetland for slihtly-polluted water treatment JI
ournal of Agro-nmironment Science, 2016, 35(11): 2154-2162
固定反硝化菌强化人工湿地处理低污染水研究
林燕,张焕杰,刘曦,俞璐,朱文颖,孔海南
上海交通大学坏境科学与工程学院,上海200240
摘要:将固定反硝化菌 Pseudomonas stutzeri(以聚乙烯醇、海藻酸钊为材料包埋固定)投加至人工湿地不同位置,进行低污染水模
拟脱氮实验,以探究固定反硝化菌的最住投加位置:采用高通量测序技术分析各系统微生物组成,并考察最优系统在低温下的脱氮
效果。结果表明,上层投加固定反硝化菌对湿地脱氮的强化效果最佳,TN与NO-N去除率分别为60.31%与64.98%;微生物多样性
指数示,投加固定反硝化菌虽降低了系统微生物丰富度,但各处理系统内主要微生物物种差异不大, Proteobacteria(变形菌」)丰
度最高,投加固定反硝化菌系统中 Nitrospira(硝化螺旋菌门)比例增大,有利丁脱氮效果的提高。在15℃条件ド,上层投加固定反硝
化菌系统的TN与NO-N去除率分别为50.86%与55.06%,高」空白湿地系统(TN与NO-N去除率为24.81%与27.53%
关键词固定化;人工湿地;脱氮;微生物多样性
中图分类号:X52文献标志码:A文章编号:1672-2043(2016)11-2154-09do:10.11654aes.2016-0540
Performance of immobilized denitrifying bacteria in constructed wetland for slightly-polluted water treatment
LIN Yan, ZHANG Huan-jie, LIU Xi, YU Lu, ZHU Wen-ying, KONG Hai-nan
(1.School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China
Abstract: Denitrifying bacteria Pseudomonas stutzeri isolated from lab were fixed with and SA and were added to dosing position(up-
per, lower, upper and lower )to simulate the release of the nitrate-rich source in low pollution water, aiming to determine the optimal dosing
position. The reasons of enhanced nitrogen removal in microbial community diversity were analyzed by using high-throughput sequencing
technology, and the effect of strengthening efficiency at low temperature was investigated. Results showed that the system dosing in upper
layer got the best performance, and the removal rates of total nitrogen and nitrate were 60.31%and 64.98%, respectively. The results of mi
crobial diversity index of each system showed that the microbial species diversity in the control system was higher than othes. But overall,
there was little difference between the four systems, and the most abundant phylum was Proteobacteria in each system. And the increased
percentage of Nitrospirain dosing systems was conducive to improve the nitrogen removal. The results showed that the removal rates of total
nitrogen and nitrate in the system dosing in upper layer were 50.86% and 55.06%, respectively, while that in the control system were
24.81%and27.53%at15%.
Keywords: immobilization; constructed wetland; denitrification: microbial diversity
收稿日期:2016-04-19
基金项目:国家水体污染控制与治理科技重大令项(2012ZX07105-003)
作者简介:林燕(1976-)女,浙江宁波人,副教授,研究方向为水污染控制与生态修复。E-mail:linyan2002@situ.edu.cn
通信作者:孔海南E-mail:hnkong@sjtu.edu.cn
万方数括